Direct Organogenesis in Local Clones of Patchouli Plant (Pogostemon cablin Benth) In Vitro

Mayerni, Reni and Warnita, Warnita and Armansyah,, Armansyah, and Chan, Sari Rukmana Okta Sagita (2020) Direct Organogenesis in Local Clones of Patchouli Plant (Pogostemon cablin Benth) In Vitro. Indonesian Journal of Crop Science (JERAMI), 3 (1). pp. 16-19. ISSN 2655-5023

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Abstract

Growth regulators that are often used in tissue culture to increase the production of secondary metabolites (organogenesis), such as shoot multiplication are cytokinin. Cytokinin growth regulators include Benzyl Amino Purine (BAP) ad kinetin. The objective of this research is to obtain the best concentration of BAP interaction with Kinetin concentration, the best concentration of BAP, and the best concentration of Kinetin that can stimulate the formation for multiplication of patchouli plants. The concentration of BAP in the in vitro growing medium varies according to the type of plant and the type of explants used. The cytokinin concentrations used ranged from 0.1-10 mg/L media. The research material used in this study was the accession of patchouli plants in West Sumatera, namely Situak and method of research is a completely randomized design. The treatment will be tested in this study consist of BAP concentrations with 5 levels ( 0.5, 1, 1,5,2,0 mg/L, and concentration of Kinetin with 2 levels ( 0.5, 1 mg/L). The basic media used were Murashige and Skoog (MS). Concentrations of 0.5 mg/L kinetin + 0.5 mg/L BAP, 0.5 mg/L kinetin + 1 mg/L BAP and 1 mg/L kinetin + 1 mg/L BAP can stimulate organogenesis (bud) in vitro. The results showed that the concentration of Kinetin and BAP influenced the growth and development of explants.

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